• Question: What method do you use when extracting DNA from protists?

    Asked by anon-329572 to Michelle on 10 Jun 2022.
    • Photo: Michelle Grey

      Michelle Grey answered on 10 Jun 2022:

      Hi Beth,

      We have been using the MagAttract kit from Qiagen but have had to make tweaks to the method for the differences in some of these species so far. The marine-based species are still being quite problematic so there will be more work required to get that right. We also had to change out the lysis buffer which came with the kit to a different one to work better on the ciliates and cryptophytes. It isn’t going to be a ‘one-size fits all’ scenario with these but that is not unusual in science, at least for me!